How to Prepare 0.4% Hyaluronic Acid Solution

To prepare a 0.4% Hyaluronic Acid solution, the Hyaluronic Acid should constitute 0.4% of the total weight of the final solution, with water making up the remaining 99.6%. A simple way to calculate this is based on the weight of the Hyaluronic Acid powder you are using. For every 1 gram of Hyaluronic Acid powder, you will need 249 grams (or milliliters, as water density is approximately 1 g/ml) of water to achieve a 0.4% concentration.

For example:

• If you use 1 gram of Hyaluronic Acid, you will need 249 grams (ml) of water.
• If you use 0.4 grams of Hyaluronic Acid, you will need 0.4 * 249 = 99.6 grams (ml) of water. The total solution weight would be 0.4g + 99.6g = 100g, where HA is 0.4% (0.4/100).

Remember to follow the recommended dissolution method: mix with water, shake, and refrigerate for about 3 hours until fully dissolved.