Measurement Wavelength (OD) for BCA Protein Content Assay Kit (ID 112753)
Asked by: Anonymous
On: October 24, 2025
Product Type:
Cosmetics
Question
What is the required measurement wavelength (Optical Density or OD) for the Protein Content Assay Kit (BCA), specifically the product with ID 112753?
Answer
The measurement wavelength (Optical Density or OD) for the Protein Content Assay Kit (BCA) is typically 562 nm.
The BCA (Bicinchoninic Acid) assay is a colorimetric method where protein reduces Cu²⁺ to Cu¹⁺ in an alkaline medium. The Cu¹⁺ then chelates with BCA to form a purple-colored reaction product that exhibits maximum absorbance at this wavelength.