Mixing Low and High Molecular Weight gamma-Polyglutamic Acid for Serum Formulation

Mixing gamma-Polyglutamic acid (LMW) and gamma-Polyglutamic acid (HMW) is a common practice to achieve multi-depth hydration, similar to using multiple molecular weights of Hyaluronic Acid. While there isn't a separate document specifically for the blend, you can follow the standard mixing guidelines for both.

Recommended Mixing Method

1. Determine Total Concentration: The recommended usage rate for Polyglutamic Acid (PGA) is 0.1% - 1% of the total formula (0.3% - 0.5% is often sufficient).
2. Calculate the Ratio: Based on your 70:30 ratio:
   • If you want 0.5% total PGA: Use 0.35% LMW and 0.15% HMW.
3. Dissolving:
   • PGA is water-soluble. Add both powders into the water phase of your serum.
   • Stir or use a high-speed mixer until the powder is completely dissolved. HMW may take slightly longer to dissolve than LMW.
4. Temperature Control: Do not exceed 60°C during the mixing process, as high heat can degrade the polymer structure.
5. pH Adjustment: PGA is generally stable in a typical skin-care pH range (approx. 5.0–7.0).

Benefits of the 70:30 Blend

• gamma-Polyglutamic acid (Low Molecule Weight) (70%): Focuses on deep skin penetration. It helps inhibit hyaluronidase (the enzyme that breaks down natural HA), increases Natural Moisturizing Factors (NMFs), and strengthens the skin from within.
• gamma-Polyglutamic acid (High Molecule Weight) (30%): Functions primarily on the skin's surface. It forms a thin, breathable film that locks in moisture, provides an immediate smoothing effect, and protects the skin barrier.

Storage and Precautions

• Storage: Keep the powders in a cool place (6-15°C) and protect them from sunlight and moisture.
• Preservation: Since this is a water-based serum, ensure you include a broad-spectrum preservative system to prevent microbial growth.