Subject: Precautions for Mixing Hyaluron + Apple Stem Cell + 4 Whitening Ingredients Serum

Hello, regarding mixing your own serum containing Hyaluron, Apple Stem Cell, and 4 whitening ingredients, there are precautions and steps to consider as follows:

Precautions and Mixing Steps

1. Will the ingredients react with each other?

• Hyaluronic Acid: Generally, Hyaluronic Acid (such as Hyaluronic Acid (Standard Molecule), Hyaluronic Acid (Small Molecule), Hyaluronic Acid (Large Molecule)) is quite compatible with most ingredients. However, attention must be paid to the appropriate pH range (should be between 3.0-9.0) and temperature not exceeding 70°C during the mixing process.
• Apple Stem Cell Extract (AppleCell™ Powder): Apple Stem Cell Extract should be mixed in the final step when the formulation temperature is below 40°C and the pH should be in the range of 3.5-6.5.
• 4 Whitening Ingredients: This is the most crucial point where you need to know the specific names of these ingredients. Each whitening ingredient (such as Vitamin C, Niacinamide, Alpha Arbutin) has significantly different properties, stability, and optimal working pH ranges. Some may react with each other or be incompatible with other ingredients. Without knowing the specific names, it's impossible to confirm compatibility and provide accurate recommendations. The advice is to check the information for each whitening ingredient you plan to use to see the appropriate pH range and mixing precautions.

2. What is the correct mixing order?

The mixing order depends on the type of formulation (e.g., water-based serum, gel) and the properties of each ingredient. Generally:

• Prepare the Base: Start with the water phase, which may contain water-soluble and heat-resistant ingredients.
• Dissolve Hyaluronic Acid: If using powdered Hyaluronic Acid, dissolve it in water according to the recommended method for each type (some require refrigeration to dissolve).
• Mix Other Ingredients: Mix other compatible and heat-resistant ingredients into the water phase.
• Cool Down: After mixing the heat-requiring parts, you must wait for the formulation temperature to cool down as specified by each ingredient.
• Add Heat-Sensitive/Final Step Ingredients: Add heat-sensitive ingredients, ingredients that must be mixed at low temperatures, or ingredients recommended for the final step. For example, Apple Stem Cell Extract (AppleCell™ Powder) should be added when the temperature is below 40°C.
• Adjust pH: After mixing all ingredients, measure the pH of the formulation and adjust it to be within the appropriate range for all active ingredients you are using, especially the whitening ingredients and Apple Stem Cell Extract (AppleCell™ Powder) which have recommended pH ranges (Apple Stem Cell Extract recommends 3.5-6.5).
• Add Preservative: Preservatives are absolutely essential when mixing your own cosmetics to prevent microbial growth. They should be added at the appropriate step according to the preservative's instructions (usually added in the final step when the formulation has cooled down).

3. What kind of water is recommended, and do you have a pH meter?

• Recommended Water: You should use clean Distilled Water or Deionized Water (DI Water) that is free from minerals and contaminants to reduce the chance of reactions with other ingredients and prevent microbial growth.
• pH Measurement: It is necessary to have a pH meter or pH strips with sufficient accuracy to measure and adjust the pH of the formulation to be within the appropriate range for all active ingredients you are using. Controlling pH is very important for the effectiveness and stability of the product.

Conclusion: Mixing multiple ingredients requires understanding the properties of each substance, especially the 4 whitening ingredients you plan to use. It is recommended to study the specific information for those ingredients further to achieve a safe, effective, and stable formulation.