Requirements for Cosmetic Subtilisin Enzyme: Stability, pH, and Activity
Asked by: Anonymous
On: October 28, 2025
Product Type:
Cosmetics
Question
I am seeking recommendations for a subtilisin enzyme suitable for cosmetic applications. The enzyme must meet the following strict specifications:
- Optimal pH range: 6.5-7.5 (Neutral)
- Grade: Cosmetic/Personal Care Grade
- Stability: Good heat stability (must be stable at 50°C for 30+ days)
- Activity: High activity (>1000 U/mg)
Answer
Based on your specific requirements for a cosmetic-grade subtilisin enzyme with high stability and activity, the available products do not fully meet all criteria.
We recommend the Protease Enzyme (ID 1017) as the closest match for cosmetic application and optimal pH, but please note the significant limitations regarding stability.
Closest Match and Details
- Product Name: Protease Enzyme (ID 1017)
- Grade: Cosmetic/Personal Care Grade (intended for skin exfoliation).
- Optimal pH: Works in a neutral pH range, aligning with your 6.5-7.5 requirement.
Limitations and Important Considerations
- Stability (50°C for 30+ days): This product does not meet the requirement for long-term stability in an aqueous solution. The enzyme's quality deteriorates rapidly (within about 30 minutes) when mixed with water.
- Formulation Requirement: It must be formulated in an anhydrous product (e.g., powder mask, cleansing powder) and mixed with water immediately before application to ensure activity.
- Subtilisin Type & Activity (>1000 U/mg): The product description does not specify if it is a Subtilisin enzyme or confirm the activity level is >1000 U/mg.
- Other Proteases: The other available stabilized protease enzymes (ID 8393, 33848) are primarily designed for laundry/stain removal and are not recommended for cosmetic skin applications. They also do not confirm the required heat stability.
