Combining Copper Peptide and Papaya Enzyme (Papain)

Based on the previous reply, Copper Peptide and Papaya Enzyme (Papain) can be used together. However, there are important formulation considerations to ensure their effectiveness and stability when combining them in a single product.

Here are the key points from the product information:

• Copper Peptide (GHK-Cu, Copper Tripeptide-1):

  • Optimal pH range for stability is 4.5-7.4.
  • Should not contain Disodium EDTA in the formula, as it will bind the Copper charge and reduce effectiveness.
  • Mix in the final step below 40°C.
  • Store in the refrigerator (4-8°C).

• Papaya Enzyme (Papain):

  • Optimal pH range for stability is 5.5-7.0.
  • Should contain 0.1-0.2% Disodium EDTA to help prevent metal ions from destroying the enzyme.
  • Avoid heat above 40°C.
  • Finished formula should be stored at a low temperature (6-15°C, ideally 4°C).
  • Enzyme efficiency decreases continuously once dissolved in water.
  • Use at a rate of 0.1-1.5% (recommended 1%), and use cautiously as it is potent.

Formulation Challenge:

The primary challenge in combining these two ingredients in a single formula is the conflicting requirement regarding Disodium EDTA. Copper Peptide's effectiveness is reduced by EDTA, while Papain's stability is improved by it. Additionally, while their optimal pH ranges overlap (5.5-7.0), achieving optimal stability for both simultaneously while managing the EDTA issue is difficult.

Recommendation:

Given the technical challenges, it is generally more practical and effective to use Copper Peptide and Papaya Enzyme (Papain) in separate products applied at different times. This avoids the direct interaction and allows each ingredient to function optimally within its ideal formulation environment.

If you choose to use Papain, remember the caution from the previous reply: use a small amount and observe your skin's response, stopping when you achieve the desired softness.