Effective Concentration (ppm) of Syn-Ake for Anti-Wrinkle Efficacy
Question
Based on previous expert advice regarding Copper Peptide (comparing 1,000ppm vs. 10,000ppm), where a usage of 1-3% was recommended for both to avoid disrupting the stability of the serum base.
I would like to inquire about the effective ppm for Syn-Ake extract. Since the raw material concentration is 2,500ppm with a recommended usage level of 1-4%, I would like to know:
- What is the actual recommended effective ppm for this ingredient?
Answer
Regarding the effective concentration (ppm) of Syn-Ake (Dipeptide Diaminobutyroyl Benzylamide Diacetate), here is the technical breakdown:
1. Effective ppm Calculation
Syn-Ake raw material typically comes in a liquid form with a concentration of 2,500 ppm. Based on the recommended usage of 1-4%, the effective concentration in your final product will be:
- At 1% usage: 25 ppm in the final formula.
- At 4% usage: 100 ppm in the final formula.
2. Recommended Efficacy Level
While 1% (25 ppm) can provide basic benefits, most clinical studies and efficacy data that demonstrate the "Botox-like" effect (reducing muscle cell contraction to smooth expression lines) are based on a usage level of 4% (100 ppm). Therefore, for maximum anti-wrinkle results, 100 ppm is considered the target effective dose.
3. Comparison with Copper Peptide Stability
The reason the recommendation for Syn-Ake is more straightforward than Copper Peptide is due to their chemical nature:
- Copper Peptide (GHK-Cu): Is a metal complex. It is highly sensitive to electrolytes, pH changes, and can significantly alter the color or stability of a serum base if used at high percentages of a high-concentration stock.
- Syn-Ake: Is a synthetic peptide that is generally more stable and does not carry the same electrolyte/metal interference issues as Copper Peptide. Thus, the 1-4% recommendation is primarily focused on dose-response efficacy rather than just protecting the formula's stability.
What I need from you to confirm:
- Are you using the standard liquid version (2500 ppm)?
- What is the target pH of your formulation? (Syn-Ake is most stable between pH 3.0 - 5.5, though it can be used up to pH 7.0).