Enzyme Mouthwash (e.q. Propolinse, 1:4 Concentrate) Usage

Regarding the use of Enzyme Mouthwash (e.q. Propolinse, 1:4 Concentrate) in your mouthwash formulation:

• Concentration: The product description recommends mixing the concentrate with distilled water or DI water at a ratio of 1:4. This means the recommended usage concentration of the concentrate in the final formula is 20%. This concentration is likely necessary to achieve the intended effect, which is the formation of the brown stain when the mouthwash reacts with proteins in saliva.

• Brown Stain Formation: The brown stain is a result of the enzymes in the concentrate reacting with proteins present in the mouth. If the brown stain did not appear when you mixed it with your general mouthwash formula, it indicates that the enzyme activity was inhibited.

• Ingredients to Avoid: The product description specifically advises avoiding adjusting the pH or adding ingredients that affect the pH of the formula. Enzymes are highly sensitive to pH levels. If the pH of your general mouthwash base was not within the optimal range for the enzymes in the concentrate, it could have denatured or inactivated them, preventing the reaction with proteins and thus the formation of the brown stain. Other ingredients in your base formula might also potentially interfere with enzyme activity, but pH is explicitly mentioned as a critical factor.

• Usage Recommendations: To ensure the product works as intended and the brown stain reaction occurs, it is crucial to use the recommended 20% concentration (1:4 dilution with distilled or DI water) and, most importantly, ensure that other ingredients in your formula do not significantly alter the pH or otherwise inhibit enzyme activity. You can add compatible ingredients like flavor, color, sweetness (Sucralose 0.01-0.03% is recommended), or cooling agents (Menthol Water Soluble) as needed, provided they do not negatively impact the enzyme activity or pH.