Formulation Compatibility

You are proposing a formulation containing Argireline (Acetyl Hexapeptide-8), Pal GHK (Matrixyl 3000), Hydrolyzed Elastin, Collagen HP, a moisturizer base, preservative, Disodium EDTA, and water.

Here's an assessment of the compatibility and mixing considerations:

• Peptides (Argireline and Pal GHK): Argireline (Acetyl Hexapeptide-8) and Pal GHK (Matrixyl 3000) are peptides commonly used together in anti-aging formulations. They are water-soluble and should be added in the final step of mixing, ensuring the temperature is below 40°C. The recommended pH range for Argireline is 3.5-6.5, and for Collagen HP it's 3.5-7.0. Matrixyl 3000 should also be mixed below 40°C. Your proposed concentrations (10% Argireline, 5% Pal GHK) are within the recommended ranges (Argireline 3-10%, Pal GHK 3-5%).
• Proteins (Hydrolyzed Elastin and Collagen HP): Hydrolyzed Elastin and Collagen HP are also water-soluble ingredients. Like the peptides, they should be mixed in the water phase or the final step, avoiding heat above 40°C. Your proposed concentrations (3-5% Hydrolyzed Elastin, 5% Collagen HP) are within the recommended ranges (Hydrolyzed Elastin 1-5%, Collagen HP 1-5%).
• Disodium EDTA: Disodium EDTA is a chelating agent that helps improve formula stability by binding to metal ions in water. It is water-soluble and should be added to the water phase before other ingredients. The recommended usage rate is 0.1-0.2%. Your proposed concentration of 0.3% is slightly higher than the typical recommendation. While it might still be functional, sticking to the recommended range is generally advisable. It is compatible within a pH range of 3.0-9.0.
• Moisturizer Base and Preservative: The compatibility of the overall formulation heavily depends on the specific moisturizer base and preservative you plan to use.
  • The moisturizer base must be compatible with water-based ingredients and allow for the incorporation of the peptides and proteins without issues like separation or degradation.
  • The preservative must be effective in the final formulation at the proposed concentration (0.5%) and compatible with all other ingredients, especially the peptides and proteins, which can be sensitive to certain preservatives or pH levels. Its effective pH range must also align with the requirements of the other ingredients (particularly the peptides and Collagen HP, which prefer a pH between 3.5 and 7.0).

Mixing Procedure:

Assuming your moisturizer base and preservative are compatible with the other ingredients and the required pH range, a general mixing procedure would be:

1. Prepare the water phase, adding Disodium EDTA first and ensuring it is fully dissolved.
2. Incorporate the moisturizer base according to its specific instructions.
3. Once the mixture has cooled to below 40°C, gently add the Argireline, Pal GHK, Hydrolyzed Elastin, and Collagen HP.
4. Add the preservative and mix thoroughly.
5. Adjust the pH of the final formulation if necessary to be within the optimal range (ideally between 3.5 and 6.5-7.0) for the peptides and proteins, while also being suitable for the preservative and moisturizer base.

Conclusion:

Mixing these ingredients is generally possible, provided that:

1. The specific moisturizer base and preservative chosen are compatible with the other ingredients.
2. The mixing temperature is kept below 40°C when adding the peptides and proteins.
3. The final pH of the formulation is within the acceptable range for all components (ideally 3.5-7.0).
4. Consider reducing the Disodium EDTA concentration to the recommended 0.1-0.2%.

It is highly recommended to perform a small test batch to check for compatibility, stability, and effectiveness before making a larger quantity.