Sample Size and Result Format for Peptide Purity Analysis (HPLC)

Asked by: apapiedaach On: November 11, 2025 Product Type: Cosmetics

Question

I plan to submit a peptide sample for Peptide Purity Analysis using High-Performance Liquid Chromatography (HPLC).

  1. Is a 10mg peptide sample in powder form sufficient for this analysis?
  2. Will the results of the Peptide Purity Analysis by HPLC be reported in percentage form?

Answer

Yes, a 10mg powder peptide sample is generally sufficient for Peptide Purity Analysis (HPLC). The standard required amount is typically 5-10mg of dry powder.

The results of the Peptide Purity Analysis by HPLC are always reported as a percentage. This percentage is calculated based on the ratio of the main peptide peak area to the total area of all detected peaks in the chromatogram.