Troubleshooting EGF Precipitation

It sounds like you are experiencing precipitation when adding Epidermal Growth Factor (EGF) to your formula containing Aloe Vera Extract, Portulaca Extract, Hyaluronic Acid, Calendula Extract, Anthemis Nobilis Flower Extract, Chamomilla recutita flower extract, Mild Preserved Eco™, and water.

EGF is a protein and its stability is highly dependent on the environment of the formulation, particularly the pH level. Based on the information for Epidermal Growth Factor (EGF, Recombinant), the optimal pH range for its stability is 3-5.

Precipitation often occurs when the pH of the final product is outside this optimal range, causing the protein to become insoluble.

To resolve this issue, please try the following steps:

1. Check the pH of your base formula: Before adding the EGF, measure the pH of your formula containing all other ingredients (Aloe Vera Extract, Portulaca Extract, Hyaluronic Acid, Calendula Extract, Anthemis Nobilis Flower Extract, Chamomilla recutita flower extract, Mild Preserved Eco™, and water).
2. Adjust the pH if necessary: If the pH is not within the 3-5 range, carefully adjust it using a suitable acid (like citric acid or lactic acid solution) or base (like sodium hydroxide solution) until it falls within the optimal range for EGF.
3. Add EGF at the correct temperature: Add the Epidermal Growth Factor (EGF, Recombinant) to your formula at the final step, ensuring the temperature is below 40°C, ideally around 25°C.
4. Mix thoroughly: Blend the mixture for at least 15 minutes after adding EGF. If it still appears cloudy or has precipitate, continue blending and let it sit for 6-12 hours to allow for complete dissolution.
5. Verify final pH: After the EGF has fully dissolved, check the pH of the final product one last time to ensure it remains within the 3-5 stability range.

Ensuring the pH is within the recommended range of 3-5 is the most critical factor for preventing EGF precipitation in your formula.