Compatibility of Flavonoids Content Assay Kit with Spectrophotometer

The Flavonoids Content Assay Kit is a colorimetric assay, meaning it measures the absorbance of the resulting colored solution at a specific wavelength (typically around 510 nm).

In principle, any instrument capable of accurately measuring absorbance at the required wavelength can be used, including a standard spectrophotometer (using cuvettes) and a microplate reader.

Key Consideration for Using a Spectrophotometer:

1. Protocol Adaptation (Scaling Volumes): The kit's protocol is often optimized for the small reaction volumes of a microplate reader (e.g., 100-300 µL per well). If you use a standard spectrophotometer, you must scale up the reaction volumes proportionally to ensure you have enough solution to fill a standard cuvette (which typically requires 1 mL or more).
2. Wavelength: Ensure your spectrophotometer can accurately read the specific wavelength required by the kit's manual.

Therefore, yes, you can use a standard spectrophotometer, but you must carefully review and potentially modify the reaction volumes in the kit's protocol to suit your cuvette size.