Mixing Techniques for Epidermal Growth Factor (EGF) in Cream Formulations
Question
The customer used Epidermal Growth Factor (EGF, Recombinant) in a cream formulation and encountered the following issues:
- The cream texture became "fluffy" and not smooth.
- The ingredient did not dissolve well, even when using a
propellermixer and continuing to blend.
What are the mixing techniques or instructions for using this ingredient to ensure a smooth texture and proper dissolution?
Answer
The issues you are experiencing with Epidermal Growth Factor (EGF, Recombinant), such as the cream becoming "fluffy" (aerated) and the ingredient not dissolving properly, can be addressed by following these specific mixing techniques:
1. Dissolving Technique
EGF is a complex of proteins (peptides and collagen) that requires time to integrate into a formula.
- Blending Time: After adding EGF to your cream base, you must blend it for at least 15 minutes.
- Resting Period: If it still appears undissolved, let the mixture rest for 6–12 hours. This allows the proteins to fully hydrate and integrate into the emulsion.
- Re-blending: After the resting period, if it is still not smooth, blend it again until completely dissolved.
2. Preventing "Fluffy" or Aerated Texture
The "fluffy" texture usually occurs because air is being whipped into the cream during the 15-minute blending process, especially when using a propeller mixer at high speeds.
- Reduce Speed: Use the lowest speed possible that still provides adequate movement.
- Propeller Position: Ensure the propeller is submerged deep enough to avoid creating a vortex that sucks in air.
- Mixing Head: If possible, use a side-sweep or anchor-type mixer for the final stage to minimize air incorporation.
3. Temperature and pH Requirements
To maintain the stability and efficacy of the Growth Factors:
- Temperature: Only add EGF when the cream has cooled down to below 40°C (ideally at room temperature, around 25°C). High heat will denature the proteins.
- pH Level: The formula should ideally have a pH between 3.0 and 5.0 for maximum stability of the EGF.
4. Compatibility
Since EGF contains proteins and collagen, it may interact with certain types of thickeners or high-electrolyte environments. Ensure your cream base is compatible with protein-based actives.
Summary of Steps:
- Prepare your cream base and cool to < 40°C.
- Add 1-5% EGF.
- Mix at a low speed for 15 minutes.
- If not smooth, leave it overnight (6-12 hours).
- Mix briefly again the next day.
