HEPES
≥99.5%(T)
- Product Code: 126964
Alias:
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES); Hydroxyethylpiperazineethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, N -(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
CAS:
7365-45-9
| Molecular Weight: | 238.3 g./mol | Molecular Formula: | C₈H₁₈N₂O₄S |
|---|---|---|---|
| EC Number: | 230-907-9 | MDL Number: | MFCD00006158 |
| Melting Point: | 234-238 °C | Boiling Point: | |
| Density: | 1.07 g/mL at 20 °C | Storage Condition: | room temperature |
Product Description:
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biochemical processes. HEPES is also commonly employed in electrophoresis, protein purification, and molecular biology techniques, where precise pH control is critical. Additionally, it is utilized in the preparation of reagents for diagnostic assays and in the study of cellular physiology, as it effectively buffers solutions in the physiological pH range (7.2–7.4). Its stability and minimal metal ion chelation make it suitable for use in experiments involving sensitive biological materials.
Product Specification:
| Test | Specification |
|---|---|
| Heavy Metals (As Pb) | 0ppm-5ppm |
| Purity (%) | 99.5%-100.5% |
| UV Absorbance | 0-0.05 |
| Water By Karl Fischer | 0%-0.5% |
| Appearance | White Powder Or Crystals |
| Infrared Spectrum | Conforms To Structure |
| Solubility In Water | Clear Colorless, 500mg/ml In Water |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 25.000 | 10-20 days | €16.36 |
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| 100.000 | 10-20 days | €48.02 |
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| 500.000 | 10-20 days | €170.69 |
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| 1000.000 | 10-20 days | €322.12 |
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| 2500.000 | 10-20 days | €690.15 |
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| 5000.000 | 10-20 days | €1,209.61 |
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HEPES
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biochemical processes. HEPES is also commonly employed in electrophoresis, protein purification, and molecular biology techniques, where precise pH control is critical. Additionally, it is utilized in the preparation of reagents for diagnostic assays and in the study of cellular physiology, as it effectively buffers solutions in the physiological pH range (7.2–7.4). Its stability and minimal metal ion chelation make it suitable for use in experiments involving sensitive biological materials.
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