HEPES

≥99.5%(T)

  • Product Code: 126964
  Alias:    N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES); Hydroxyethylpiperazineethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, N -(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
  CAS:    7365-45-9
Molecular Weight: 238.3 g./mol Molecular Formula: C₈H₁₈N₂O₄S
EC Number: 230-907-9 MDL Number: MFCD00006158
Melting Point: 234-238 °C Boiling Point:
Density: 1.07 g/mL at 20 °C Storage Condition: room temperature
Product Description: HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biochemical processes. HEPES is also commonly employed in electrophoresis, protein purification, and molecular biology techniques, where precise pH control is critical. Additionally, it is utilized in the preparation of reagents for diagnostic assays and in the study of cellular physiology, as it effectively buffers solutions in the physiological pH range (7.2–7.4). Its stability and minimal metal ion chelation make it suitable for use in experiments involving sensitive biological materials.
Product Specification:
Test Specification
Heavy Metals (As Pb) 0ppm-5ppm
Purity (%) 99.5%-100.5%
UV Absorbance 0-0.05
Water By Karl Fischer 0%-0.5%
Appearance White Powder Or Crystals
Infrared Spectrum Conforms To Structure
Solubility In Water Clear Colorless, 500mg/ml In Water
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
25.000 10-20 days €16.36
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100.000 10-20 days €48.02
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500.000 10-20 days €170.69
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1000.000 10-20 days €322.12
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2500.000 10-20 days €690.15
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5000.000 10-20 days €1,209.61
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HEPES
HEPES is widely used as a buffering agent in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly favored in cell and tissue culture applications because it is non-toxic to cells and does not interfere with biochemical processes. HEPES is also commonly employed in electrophoresis, protein purification, and molecular biology techniques, where precise pH control is critical. Additionally, it is utilized in the preparation of reagents for diagnostic assays and in the study of cellular physiology, as it effectively buffers solutions in the physiological pH range (7.2–7.4). Its stability and minimal metal ion chelation make it suitable for use in experiments involving sensitive biological materials.
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