Laemmli's (SDS-Sample) Buffer
6X, non-reductive
- Product Code: 66726
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| Density: | Storage Condition: | 2-8°C |
Product Description:
Laemmli's (SDS-Sample) Buffer is widely used in molecular biology and biochemistry for preparing protein samples for SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its primary application is to denature proteins, ensuring they unfold and adopt a linear structure, which is essential for accurate separation based on molecular weight. The buffer contains SDS, which binds to proteins and imparts a uniform negative charge, allowing them to migrate consistently in an electric field. Additionally, it includes a reducing agent, typically beta-mercaptoethanol or dithiothreitol, which breaks disulfide bonds, further aiding in protein denaturation. Laemmli's Buffer also contains glycerol, which helps samples sink into the wells of the gel, and a tracking dye, such as bromophenol blue, to monitor the progress of electrophoresis. This buffer is a critical component in protein analysis, enabling researchers to study protein size, purity, and expression levels effectively.
Product Specification:
| Test | Specification |
|---|---|
| APPEARANCE | Liquid |
| SDS | 6 |
| Glycerol | 20 |
| Infrared spectrum | 30 |
| 2-mercaptoethanol | 15 |
| Bromophenol blue | 0.006 |
| Tris HCl | 0.1875 mol/L |
| Smell | Pungent odor |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 25.000 | 10-20 days | $150.28 |
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Laemmli's (SDS-Sample) Buffer
Laemmli's (SDS-Sample) Buffer is widely used in molecular biology and biochemistry for preparing protein samples for SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its primary application is to denature proteins, ensuring they unfold and adopt a linear structure, which is essential for accurate separation based on molecular weight. The buffer contains SDS, which binds to proteins and imparts a uniform negative charge, allowing them to migrate consistently in an electric field. Additionally, it includes a reducing agent, typically beta-mercaptoethanol or dithiothreitol, which breaks disulfide bonds, further aiding in protein denaturation. Laemmli's Buffer also contains glycerol, which helps samples sink into the wells of the gel, and a tracking dye, such as bromophenol blue, to monitor the progress of electrophoresis. This buffer is a critical component in protein analysis, enabling researchers to study protein size, purity, and expression levels effectively.
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