Ethidium bromide(EB)
≥95% (HPLC)
- Product Code: 98375
Alias:
Ethidium bromide; 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, ethidium bromide
CAS:
1239-45-8
| Molecular Weight: | 394.31 g./mol | Molecular Formula: | C₂₁H₂₀BrN₃ |
|---|---|---|---|
| EC Number: | MDL Number: | MFCD00011724 | |
| Melting Point: | 260-262 °C (dec.)(lit.) | Boiling Point: | |
| Density: | Storage Condition: | room temperature |
Product Description:
Ethidium bromide is widely used in molecular biology for staining nucleic acids, particularly in agarose gel electrophoresis. It intercalates between DNA base pairs, allowing for the visualization of DNA bands under UV light. This application is crucial for analyzing DNA fragments, verifying PCR products, and assessing DNA integrity. Additionally, it is employed in fluorescence microscopy to study chromosome structures and in flow cytometry for DNA content analysis. However, due to its mutagenic properties, safer alternatives like SYBR Green are increasingly preferred. Proper handling and disposal protocols are essential when using ethidium bromide to minimize health and environmental risks.
Product Specification:
| Test | Specification |
|---|---|
| LOSS ON DRYING | 0 5% |
| Purity | 95 100% |
| APPEARANCE | RED TO VERY DARK RED OR DARK PURPLE POWDER |
| INFRARED SPECTRUM | Conforms to Structure |
| SOLUBILITY | RED TO VERY DARK RED,C =10MG/ML; WATER |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | $30.60 |
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| 5.000 | 10-20 days | $119.41 |
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| 25.000 | 10-20 days | $564.06 |
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Ethidium bromide(EB)
Ethidium bromide is widely used in molecular biology for staining nucleic acids, particularly in agarose gel electrophoresis. It intercalates between DNA base pairs, allowing for the visualization of DNA bands under UV light. This application is crucial for analyzing DNA fragments, verifying PCR products, and assessing DNA integrity. Additionally, it is employed in fluorescence microscopy to study chromosome structures and in flow cytometry for DNA content analysis. However, due to its mutagenic properties, safer alternatives like SYBR Green are increasingly preferred. Proper handling and disposal protocols are essential when using ethidium bromide to minimize health and environmental risks.
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