6-Chloro-3-indolyl β-D-Galactopyranoside
≥98%
- Product Code: 103765
CAS:
138182-21-5
| Molecular Weight: | 329.73 g./mol | Molecular Formula: | C₁₄H₁₆ClNO₆ |
|---|---|---|---|
| EC Number: | MDL Number: | MFCD00467206 | |
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | -20°C |
Product Description:
Used as a substrate in molecular biology for detecting β-galactosidase activity. It is commonly applied in blue-white screening to identify recombinant bacteria. When cleaved by β-galactosidase, it produces an insoluble blue product, allowing for visual differentiation between colonies with and without the enzyme. This makes it valuable in cloning experiments, gene expression studies, and mutagenesis assays. It is also utilized in histochemistry and biochemistry research to localize and quantify β-galactosidase in cells and tissues.
Product Specification:
| Test | Specification |
|---|---|
| Appearance | White To Light Yellow Powder To Crystal |
| Purity (%) | 98-100 |
| Specific Rotation [a]20/D(C=1, MeOH) | -47.0--43.0 |
| Infrared Spectrum | Conforms To Structure |
| Beta-Galactosidase Detection Test | To Pass Test |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.020 | 10-20 days | ฿4,680.00 |
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| 0.100 | 10-20 days | ฿12,680.00 |
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| 0.500 | 10-20 days | ฿29,460.00 |
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6-Chloro-3-indolyl β-D-Galactopyranoside
Used as a substrate in molecular biology for detecting β-galactosidase activity. It is commonly applied in blue-white screening to identify recombinant bacteria. When cleaved by β-galactosidase, it produces an insoluble blue product, allowing for visual differentiation between colonies with and without the enzyme. This makes it valuable in cloning experiments, gene expression studies, and mutagenesis assays. It is also utilized in histochemistry and biochemistry research to localize and quantify β-galactosidase in cells and tissues.
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