Propidium iodide
biotech grade
- Product Code: 110699
CAS:
25535-16-4
| Molecular Weight: | 668.39 g./mol | Molecular Formula: | C₂₇H₃₄I₂N₄ |
|---|---|---|---|
| EC Number: | 247-081-0 | MDL Number: | MFCD00011921 |
| Melting Point: | 220-225 °C (dec.)(lit.) | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
Propidium iodide is widely used in biological research for staining cells to assess cell viability. It is particularly effective in distinguishing between live and dead cells due to its ability to penetrate compromised cell membranes, which are characteristic of dead or dying cells. Once inside the cell, it binds to DNA by intercalating between the bases, emitting a strong red fluorescence when excited by specific wavelengths of light. This property makes it a valuable tool in flow cytometry and fluorescence microscopy for analyzing cell populations and determining the percentage of dead cells in a sample. Additionally, it is employed in studies involving apoptosis and necrosis to monitor cell death processes. Its use is also common in plant biology for analyzing pollen viability and in microbiology for evaluating bacterial cell integrity.
Product Specification:
| Test | Specification |
|---|---|
| Purity (HPLC) | 94-100 |
| Water By Karl Fischer | 0-5 |
| Appearance | Faint Red To Very Dark Red Powder |
| Infrared Spectrum | Conforms To Structure |
| Solubility | Red Clear Solution (1 Mg/Ml, H2O) |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.005 | 10-20 days | ฿1,150.00 |
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| 0.025 | 10-20 days | ฿2,990.00 |
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| 0.100 | 10-20 days | ฿8,230.00 |
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| 0.500 | 10-20 days | ฿29,860.00 |
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| 1.000 | 10-20 days | ฿53,550.00 |
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Propidium iodide
Propidium iodide is widely used in biological research for staining cells to assess cell viability. It is particularly effective in distinguishing between live and dead cells due to its ability to penetrate compromised cell membranes, which are characteristic of dead or dying cells. Once inside the cell, it binds to DNA by intercalating between the bases, emitting a strong red fluorescence when excited by specific wavelengths of light. This property makes it a valuable tool in flow cytometry and fluorescence microscopy for analyzing cell populations and determining the percentage of dead cells in a sample. Additionally, it is employed in studies involving apoptosis and necrosis to monitor cell death processes. Its use is also common in plant biology for analyzing pollen viability and in microbiology for evaluating bacterial cell integrity.
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