HEPES Lysis Buffer with NP-40
- Product Code: 97769
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| Density: | Storage Condition: | 2-8°C |
Product Description:
HEPES Lysis Buffer with NP-40 is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its application includes:
- **Protein Extraction**: Efficiently disrupts cell membranes to release intracellular proteins while maintaining protein stability and function.
- **Immunoprecipitation**: Commonly used as a lysis buffer to prepare cell extracts for immunoprecipitation assays, ensuring minimal interference with antibody-antigen interactions.
- **Western Blotting**: Provides a suitable environment for extracting proteins for subsequent separation and detection in Western blotting experiments.
- **Enzyme Activity Studies**: Preserves enzyme activity during extraction, making it ideal for studying enzymatic functions and kinetics.
- **Subcellular Fractionation**: Helps isolate specific cellular components, such as organelles, for detailed analysis.
- **Cell Signaling Studies**: Maintains the integrity of signaling molecules, making it useful for studying phosphorylation and other post-translational modifications.
Its mild detergent properties and buffering capacity make it a versatile choice for various experimental protocols.
Product Specification:
| Test | Specification |
|---|---|
| Performance test | PASS |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 100.000 | 10-20 days | $69.91 |
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| 250.000 | 10-20 days | $144.31 |
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HEPES Lysis Buffer with NP-40
HEPES Lysis Buffer with NP-40 is widely used in molecular biology and biochemistry for cell lysis and protein extraction. Its application includes:
- **Protein Extraction**: Efficiently disrupts cell membranes to release intracellular proteins while maintaining protein stability and function.
- **Immunoprecipitation**: Commonly used as a lysis buffer to prepare cell extracts for immunoprecipitation assays, ensuring minimal interference with antibody-antigen interactions.
- **Western Blotting**: Provides a suitable environment for extracting proteins for subsequent separation and detection in Western blotting experiments.
- **Enzyme Activity Studies**: Preserves enzyme activity during extraction, making it ideal for studying enzymatic functions and kinetics.
- **Subcellular Fractionation**: Helps isolate specific cellular components, such as organelles, for detailed analysis.
- **Cell Signaling Studies**: Maintains the integrity of signaling molecules, making it useful for studying phosphorylation and other post-translational modifications.
Its mild detergent properties and buffering capacity make it a versatile choice for various experimental protocols.
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