Proteinase K
≥40 U/mg,NGS
- Product Code: 99071
Alias:
Proteinase K ; Proteinase K
CAS:
39450-01-6
Molecular Weight: | Molecular Formula: | ||
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EC Number: | 254-457-8 | MDL Number: | MFCD00132129 |
Melting Point: | Boiling Point: | ||
Density: | Storage Condition: | -20℃ |
Product Description:
Proteinase K is widely used in molecular biology for its ability to digest proteins and remove contaminants from nucleic acid preparations. It is particularly effective in breaking down nucleases that could degrade DNA or RNA during extraction, ensuring high-quality genetic material for downstream applications like PCR, sequencing, and cloning. In histology, it is employed for antigen retrieval, enhancing the detection of proteins in tissue samples by unmasking epitopes. Additionally, it is used in the preparation of samples for mass spectrometry, where it aids in protein digestion for peptide analysis. Its robust activity in the presence of denaturants like SDS makes it a versatile tool in various biochemical and diagnostic protocols.
Product Specification:
Test | Specification |
---|---|
APPEARANCE | White to Off White Powder |
ActivityUmg solid | 40 |
RNase | Not detected |
DNASE | Not detected |
Infrared spectrum | Conforms to Structure |
NMR | Conforms to Structure |
Sizes / Availability / Pricing:
Size (g) | Availability | Price | Quantity |
---|---|---|---|
0.100 | 10-20 days | ฿3,660.00 |
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0.500 | 10-20 days | ฿15,680.00 |
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1.000 | 10-20 days | ฿28,600.00 |
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5.000 | 10-20 days | ฿108,800.00 |
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Proteinase K
Proteinase K is widely used in molecular biology for its ability to digest proteins and remove contaminants from nucleic acid preparations. It is particularly effective in breaking down nucleases that could degrade DNA or RNA during extraction, ensuring high-quality genetic material for downstream applications like PCR, sequencing, and cloning. In histology, it is employed for antigen retrieval, enhancing the detection of proteins in tissue samples by unmasking epitopes. Additionally, it is used in the preparation of samples for mass spectrometry, where it aids in protein digestion for peptide analysis. Its robust activity in the presence of denaturants like SDS makes it a versatile tool in various biochemical and diagnostic protocols.
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