L-Leucine-p-nitroanilide
≥98%
- Product Code: 105247
Alias:
L-leucine-4-nitroaniline
CAS:
4178-93-2
| Molecular Weight: | 251.28 g./mol | Molecular Formula: | C₁₂H₁₇N₃O₃ |
|---|---|---|---|
| EC Number: | 224-047-3 | MDL Number: | MFCD00063681 |
| Melting Point: | 88-90 °C | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
L-Leucine-p-nitroanilide is primarily used as a substrate in enzymatic assays to study the activity of proteases, particularly leucine aminopeptidases. These enzymes play a crucial role in breaking down proteins and peptides, and the compound helps in detecting and quantifying their activity. When the enzyme cleaves the amide bond in L-Leucine-p-nitroanilide, it releases p-nitroaniline, which is a yellow-colored product. This color change can be easily measured using spectrophotometry, providing a straightforward method to monitor enzyme kinetics and efficiency. Its application is significant in biochemical research, drug development, and clinical diagnostics, where understanding protease activity is essential.
Product Specification:
| Test | Specification |
|---|---|
| Melting Point | 89-90 |
| Purity (%) | 98-100 |
| Specific Rotation [A]25/D(C = 2 In Meoh) | 20-23.7 |
| Appearance | White To Cream To Pale Yellow Crystals Or Powder Or Crystalline Powder |
| Infrared Spectrometry | Conforms To Structure |
| Solubility In EtOH | Light Yellow And Yellow-Green Clear, 50 Mg/Ml |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | $120.54 |
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| 5.000 | 10-20 days | $517.33 |
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L-Leucine-p-nitroanilide
L-Leucine-p-nitroanilide is primarily used as a substrate in enzymatic assays to study the activity of proteases, particularly leucine aminopeptidases. These enzymes play a crucial role in breaking down proteins and peptides, and the compound helps in detecting and quantifying their activity. When the enzyme cleaves the amide bond in L-Leucine-p-nitroanilide, it releases p-nitroaniline, which is a yellow-colored product. This color change can be easily measured using spectrophotometry, providing a straightforward method to monitor enzyme kinetics and efficiency. Its application is significant in biochemical research, drug development, and clinical diagnostics, where understanding protease activity is essential.
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