RIPA Lysis Buffer(Strong)
BR, no inhibitor
- Product Code: 111562
Alias:
RIPA lysate (strong, no inhibitor)
| Molecular Weight: | Molecular Formula: | ||
|---|---|---|---|
| EC Number: | MDL Number: | ||
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | -20ºC |
Product Description:
RIPA Lysis Buffer (Strong) is widely used in molecular biology and biochemistry for the efficient extraction of proteins from cells and tissues. Its strong formulation is particularly effective in breaking down cell membranes and nuclear membranes, making it suitable for isolating both cytoplasmic and nuclear proteins. This buffer is commonly employed in techniques such as Western blotting, immunoprecipitation, and protein assays, where high-quality protein samples are essential. Additionally, it helps in solubilizing membrane-bound proteins, which can be challenging to extract with milder buffers. The inclusion of detergents and inhibitors in the buffer ensures the preservation of protein integrity by preventing degradation and maintaining biological activity during the extraction process.
Product Specification:
| Test | Specification |
|---|---|
| Appearance | Colorless Liquid |
| Protease Inhibitor | None |
| Phosphatase Inhibitor | None |
| Infrared Spectrum | Conforms To Structure |
| Tris | 50mM |
| Nacl | 150mM |
| Triton X-100 | 1% |
| Sodium Deoxycholate | 1% |
| Sds | 0.1% |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 50.000 | 10-20 days | £38.00 |
+
-
|
| 250.000 | 10-20 days | £128.46 |
+
-
|
| 1000.000 | 10-20 days | £367.30 |
+
-
|
RIPA Lysis Buffer(Strong)
RIPA Lysis Buffer (Strong) is widely used in molecular biology and biochemistry for the efficient extraction of proteins from cells and tissues. Its strong formulation is particularly effective in breaking down cell membranes and nuclear membranes, making it suitable for isolating both cytoplasmic and nuclear proteins. This buffer is commonly employed in techniques such as Western blotting, immunoprecipitation, and protein assays, where high-quality protein samples are essential. Additionally, it helps in solubilizing membrane-bound proteins, which can be challenging to extract with milder buffers. The inclusion of detergents and inhibitors in the buffer ensures the preservation of protein integrity by preventing degradation and maintaining biological activity during the extraction process.
| Mechanism | - |
| Appearance | - |
| Longevity | - |
| Strength | - |
| Storage | - |
| Shelf Life | - |
| Allergen(s) | - |
| Dosage (Range) | - |
| Recommended Dosage | - |
| Dosage (Per Day) | - |
| Recommended Dosage (Per Day) | - |
| Mix Method | - |
| Heat Resistance | - |
| Stable in pH range | - |
| Solubility | - |
| Product Types | - |
| INCI | - |
Cart
No products
Subtotal:
£0.00
£0.00
Total :