SDS Lysis Buffer
BR
- Product Code: 111565
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|---|---|---|---|
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| Density: | Storage Condition: | -20ºC |
Product Description:
SDS Lysis Buffer is widely used in molecular biology and biochemistry for the efficient disruption of cell membranes and solubilization of proteins. It is particularly essential in the preparation of protein samples for SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), where it helps denature proteins and impart a uniform negative charge, enabling separation based on molecular weight. The buffer is also employed in DNA and RNA extraction protocols to break down cellular structures and release nucleic acids. Additionally, it is utilized in Western blotting for lysing cells and extracting proteins, ensuring that the target proteins are accessible for detection. Its ability to disrupt non-covalent bonds in proteins makes it a valuable tool in studying protein structure and function.
Product Specification:
| Test | Specification |
|---|---|
| Appearance | Colorless Transparent Liquid |
| Density (20 Degree Celsius) | 1.00g/ml-1.04g/ml |
| PH (25 Degree Celsius) | 7.5-8.5 |
| Sediment | nothing |
| Bubble | Shaking With Bubbles |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 50.000 | 10-20 days | ฿1,160.00 |
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| 250.000 | 10-20 days | ฿4,680.00 |
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| 1000.000 | 10-20 days | ฿18,000.00 |
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SDS Lysis Buffer
SDS Lysis Buffer is widely used in molecular biology and biochemistry for the efficient disruption of cell membranes and solubilization of proteins. It is particularly essential in the preparation of protein samples for SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), where it helps denature proteins and impart a uniform negative charge, enabling separation based on molecular weight. The buffer is also employed in DNA and RNA extraction protocols to break down cellular structures and release nucleic acids. Additionally, it is utilized in Western blotting for lysing cells and extracting proteins, ensuring that the target proteins are accessible for detection. Its ability to disrupt non-covalent bonds in proteins makes it a valuable tool in studying protein structure and function.
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