4-Nitrophenyl (E)-3-(4-nitrophenyl)acrylate
95%
- Product Code: 181124
CAS:
27019-24-5
| Molecular Weight: | 314.25 g./mol | Molecular Formula: | C₁₅H₁₀N₂O₆ |
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| EC Number: | MDL Number: | ||
| Melting Point: | 181-183 °C | Boiling Point: | 503.0±43.0 °C(Predicted) |
| Density: | 1.430±0.06 g/cm3(Predicted) | Storage Condition: | Room temperature, seal, dry |
Product Description:
Used as a chromogenic substrate in enzymatic assays, particularly for detecting esterase and lipase activity. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which produces a yellow color detectable at 400–410 nm, enabling real-time monitoring of enzyme activity. Its dual nitrophenyl groups enhance electrophilicity, making it highly reactive toward nucleophilic attack by serine residues in hydrolases. Commonly employed in high-throughput screening, diagnostic kits, and biochemical research to quantify enzyme kinetics and inhibition. Also utilized in developing biosensors and studying enzyme mechanisms due to its clear colorimetric response.
Sizes / Availability / Pricing:
| Size | Availability | Price | Quantity |
|---|---|---|---|
| 250mg | 10-20 days | ฿2,730.00 |
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| 1g | 10-20 days | ฿7,350.00 |
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| 100mg | 10-20 days | ฿1,600.00 |
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4-Nitrophenyl (E)-3-(4-nitrophenyl)acrylate
Used as a chromogenic substrate in enzymatic assays, particularly for detecting esterase and lipase activity. Upon enzymatic hydrolysis, it releases 4-nitrophenol, which produces a yellow color detectable at 400–410 nm, enabling real-time monitoring of enzyme activity. Its dual nitrophenyl groups enhance electrophilicity, making it highly reactive toward nucleophilic attack by serine residues in hydrolases. Commonly employed in high-throughput screening, diagnostic kits, and biochemical research to quantify enzyme kinetics and inhibition. Also utilized in developing biosensors and studying enzyme mechanisms due to its clear colorimetric response.
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