Phosphoric acid mono-(4-nitro-phenyl)este

98%

  • Product Code: 227486
  CAS:    330-13-2
Molecular Weight: 219.08 g./mol Molecular Formula: C₆H₆NO₆P
EC Number: MDL Number: MFCD00067095
Melting Point: Boiling Point:
Density: Storage Condition: Room temperature
Product Description: Used as a chromogenic substrate in enzymatic assays, particularly for detecting alkaline phosphatase activity. When cleaved by the enzyme, it releases 4-nitrophenol, which produces a yellow color measurable at 405 nm in spectrophotometric analysis. This makes it valuable in clinical diagnostics, immunoassays (such as ELISA), and biochemical research for quantifying enzyme activity. Its color development is stable and sensitive, allowing detection of low enzyme concentrations. Also employed in high-throughput screening and kinetic studies due to its reliable reaction kinetics and ease of detection.
Sizes / Availability / Pricing:
Size Availability Price Quantity
25mg 10-20 days ฿1,500.00
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100mg 10-20 days ฿4,200.00
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250mg 10-20 days ฿7,200.00
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1g 10-20 days ฿17,200.00
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5g 10-20 days ฿46,000.00
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Phosphoric acid mono-(4-nitro-phenyl)este
Used as a chromogenic substrate in enzymatic assays, particularly for detecting alkaline phosphatase activity. When cleaved by the enzyme, it releases 4-nitrophenol, which produces a yellow color measurable at 405 nm in spectrophotometric analysis. This makes it valuable in clinical diagnostics, immunoassays (such as ELISA), and biochemical research for quantifying enzyme activity. Its color development is stable and sensitive, allowing detection of low enzyme concentrations. Also employed in high-throughput screening and kinetic studies due to its reliable reaction kinetics and ease of detection.
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