Tris-HCl Buffer
0.1M,pH7.5
- Product Code: 238670
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| Density: | Storage Condition: | 2-8°C |
Product Description:
Tris-HCl buffer is widely used in biological and biochemical research due to its effective buffering range in the physiological pH region (approximately pH 7.0–9.0). It is commonly employed in nucleic acid and protein experiments, including DNA and RNA electrophoresis, where it helps maintain stable pH conditions during gel running. It is a key component in many cell lysis and extraction buffers, ensuring proteins remain stable during isolation. Tris-HCl is also used in enzyme assays, where pH stability is critical for maintaining enzyme activity. Additionally, it serves as a running buffer in chromatography and in the preparation of samples for Western blotting and PCR. Its low reactivity with biological molecules and minimal interference with biochemical reactions make it a preferred choice in molecular biology laboratories.
Sizes / Availability / Pricing:
| Size | Availability | Price | Quantity |
|---|---|---|---|
| 100ml | 10-20 days | €26.09 |
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| 250ml | 10-20 days | €52.18 |
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| 1L | 10-20 days | €156.80 |
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Tris-HCl Buffer
Tris-HCl buffer is widely used in biological and biochemical research due to its effective buffering range in the physiological pH region (approximately pH 7.0–9.0). It is commonly employed in nucleic acid and protein experiments, including DNA and RNA electrophoresis, where it helps maintain stable pH conditions during gel running. It is a key component in many cell lysis and extraction buffers, ensuring proteins remain stable during isolation. Tris-HCl is also used in enzyme assays, where pH stability is critical for maintaining enzyme activity. Additionally, it serves as a running buffer in chromatography and in the preparation of samples for Western blotting and PCR. Its low reactivity with biological molecules and minimal interference with biochemical reactions make it a preferred choice in molecular biology laboratories.
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