Bis-Tris
99% biotech grade
- Product Code: 50760
CAS:
6976-37-0
Molecular Weight: | 209.24 g./mol | Molecular Formula: | C₈H₁₉NO₅ |
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EC Number: | 230-237-7 | MDL Number: | MFCD00002853 |
Melting Point: | 104 °C(lit.) | Boiling Point: | |
Density: | Storage Condition: | room temperature |
Product Description:
Bis-Tris is widely used as a buffering agent in biochemical and molecular biology applications. Its effective pH range of 5.8 to 7.2 makes it suitable for maintaining stable pH conditions in various experimental setups, such as protein purification and electrophoresis. It is particularly favored in gel electrophoresis for its low conductivity, which minimizes heat generation and improves resolution. Additionally, Bis-Tris is used in cell culture media to maintain physiological pH levels, supporting cell growth and viability. Its compatibility with metal ions also makes it useful in studies involving metalloproteins and enzymatic reactions.
Product Specification:
Test | Specification |
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ASSAY | 99-101 |
PH1WATER | 9.5-11 |
A 1 cm0.1 M in water280nm | 0-0.04 |
A 1 cm0.1 M in water340nm | 0-0.02 |
WATER BY KARL FISCHER | 0-1 |
APPEARANCE | White crystalline powder |
SOLUBILITY IN WATER 0.1M | Clear colorless liquid |
Sizes / Availability / Pricing:
Size (g) | Availability | Price | Quantity |
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25.000 | 10-20 days | ฿790.00 |
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100.000 | 10-20 days | ฿2,960.00 |
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500.000 | 10-20 days | ฿12,600.00 |
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Bis-Tris
Bis-Tris is widely used as a buffering agent in biochemical and molecular biology applications. Its effective pH range of 5.8 to 7.2 makes it suitable for maintaining stable pH conditions in various experimental setups, such as protein purification and electrophoresis. It is particularly favored in gel electrophoresis for its low conductivity, which minimizes heat generation and improves resolution. Additionally, Bis-Tris is used in cell culture media to maintain physiological pH levels, supporting cell growth and viability. Its compatibility with metal ions also makes it useful in studies involving metalloproteins and enzymatic reactions.
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