5-Bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
>99.0% Biotech Grade
- Product Code: 52335
Alias:
X-Gal
CAS:
7240-90-6
| Molecular Weight: | 408.63 g./mol | Molecular Formula: | C₁₄H₁₅BrClNO₆ |
|---|---|---|---|
| EC Number: | 230-640-8 | MDL Number: | MFCD00005666 |
| Melting Point: | 230 °C | Boiling Point: | |
| Density: | Storage Condition: | −20°C |
Product Description:
Used extensively in molecular biology as a substrate for detecting β-galactosidase activity. When cleaved by the enzyme, it produces an insoluble blue product, making it valuable for visualizing gene expression in techniques like X-gal staining. Commonly employed in cloning experiments to identify recombinant bacteria containing plasmids with lacZ gene inserts. Also utilized in histochemistry and cell biology to study cellular localization and activity of β-galactosidase in tissues and cells. Its sensitivity and specificity make it a preferred choice in research involving reporter gene assays and genetic engineering.
Product Specification:
| Test | Specification |
|---|---|
| 1:1 | -63--60 |
| Appearance | White to off-white crystalline powder |
| Solubility 5 wv in DMF | Soluble in Dimethylformamide |
| Identification | The IR spectrum of sample should be Concordant with that of working standard |
| DMF:METHANOL 1:1 | ClearColorless to Light Yellow Solution |
| Moisture Content by KF | 1.0 w/w |
| Thin Layer Chromatograph | Should be Single Spot |
| Purity by HPLC | 99 |
| Assay by HPLC On anhydrous basis | 98.00 w/w |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | ฿2,960.00 |
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| 5.000 | 10-20 days | ฿12,680.00 |
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5-Bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
Used extensively in molecular biology as a substrate for detecting β-galactosidase activity. When cleaved by the enzyme, it produces an insoluble blue product, making it valuable for visualizing gene expression in techniques like X-gal staining. Commonly employed in cloning experiments to identify recombinant bacteria containing plasmids with lacZ gene inserts. Also utilized in histochemistry and cell biology to study cellular localization and activity of β-galactosidase in tissues and cells. Its sensitivity and specificity make it a preferred choice in research involving reporter gene assays and genetic engineering.
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| Stable in pH range | - |
| Solubility | - |
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