4-Nitrophenyl phosphate bis(cyclohexylammonium) salt phosphatase substrate

97%

  • Product Code: 60609
  CAS:    52483-84-8
Molecular Weight: 417.45 g./mol Molecular Formula: C₁₈H₃₂N₃O₆P
EC Number: MDL Number: MFCD00013173
Melting Point: 199-200 °C(lit.) Boiling Point: 457.8 °C at 760 mmHg (lit.)
Density: Storage Condition: −20°C
Product Description: This chemical is widely used as a substrate in enzymatic assays to measure the activity of alkaline phosphatase enzymes. It is particularly valuable in biochemical and biomedical research for detecting and quantifying enzyme activity in various biological samples. When the enzyme acts on this substrate, it produces a yellow-colored product, 4-nitrophenol, which can be easily measured using spectrophotometry. This makes it a convenient tool for high-throughput screening and diagnostic applications, such as monitoring liver function or detecting bone disorders. Additionally, it is employed in ELISA (Enzyme-Linked Immunosorbent Assay) techniques for detecting antibodies or antigens in immunology studies. Its stability and sensitivity make it a reliable choice for laboratory diagnostics and research purposes.
Product Specification:
Test Specification
APPEARANCE Light Yellow Powder
PURITY 96.5-100
Infrared spectrum Conforms to Structure
NMR Conforms to Structure
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
1.000 10-20 days ฿2,560.00
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5.000 10-20 days ฿10,250.00
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4-Nitrophenyl phosphate bis(cyclohexylammonium) salt phosphatase substrate
This chemical is widely used as a substrate in enzymatic assays to measure the activity of alkaline phosphatase enzymes. It is particularly valuable in biochemical and biomedical research for detecting and quantifying enzyme activity in various biological samples. When the enzyme acts on this substrate, it produces a yellow-colored product, 4-nitrophenol, which can be easily measured using spectrophotometry. This makes it a convenient tool for high-throughput screening and diagnostic applications, such as monitoring liver function or detecting bone disorders. Additionally, it is employed in ELISA (Enzyme-Linked Immunosorbent Assay) techniques for detecting antibodies or antigens in immunology studies. Its stability and sensitivity make it a reliable choice for laboratory diagnostics and research purposes.
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