5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt
≥98%
- Product Code: 61256
CAS:
129541-41-9
| Molecular Weight: | 444.59 g./mol | Molecular Formula: | C₁₄H₁₂BrClNNaO₇ |
|---|---|---|---|
| EC Number: | MDL Number: | MFCD00135782 | |
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | 2-8°C |
Product Description:
This chemical is widely used as a substrate in biochemical assays, particularly for the detection of β-glucuronidase enzyme activity. It is commonly employed in microbiology and molecular biology to identify and quantify bacterial colonies, especially Escherichia coli, which express β-glucuronidase. When the enzyme cleaves the substrate, it produces an insoluble blue-green indigo derivative, making it a valuable tool for visual or spectrophotometric detection. It is also utilized in histochemistry and immunohistochemistry for locating enzyme activity in tissue samples. Additionally, it plays a role in environmental monitoring to assess water quality by detecting fecal contamination. Its sensitivity and specificity make it a key component in diagnostic and research applications.
Product Specification:
| Test | Specification |
|---|---|
| APPEARANCE | White to Almost white powder to crystal |
| PURITY | 97.5-100 |
| Infrared spectrum | Conforms to Structure |
| NMR | Conforms to Structure |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.010 | 10-20 days | €19.79 |
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| 0.100 | 10-20 days | €76.77 |
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| 0.500 | 10-20 days | €317.11 |
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5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt
This chemical is widely used as a substrate in biochemical assays, particularly for the detection of β-glucuronidase enzyme activity. It is commonly employed in microbiology and molecular biology to identify and quantify bacterial colonies, especially Escherichia coli, which express β-glucuronidase. When the enzyme cleaves the substrate, it produces an insoluble blue-green indigo derivative, making it a valuable tool for visual or spectrophotometric detection. It is also utilized in histochemistry and immunohistochemistry for locating enzyme activity in tissue samples. Additionally, it plays a role in environmental monitoring to assess water quality by detecting fecal contamination. Its sensitivity and specificity make it a key component in diagnostic and research applications.
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