NP40 Lysis Buffer

  • Product Code: 64956
  Alias:    NP-40 lysate
Molecular Weight: Molecular Formula:
EC Number: MDL Number:
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Density: Storage Condition: 2-8°C
Product Description: NP40 Lysis Buffer is widely used in molecular biology and biochemistry for cell lysis and protein extraction. It is particularly effective for isolating proteins from cell membranes and cytoplasm due to its ability to disrupt lipid bilayers while maintaining protein integrity. This buffer is commonly employed in immunoprecipitation, Western blotting, and enzyme activity assays, as it solubilizes proteins without denaturing them. It is also suitable for extracting nuclear and cytoplasmic proteins from cultured cells, making it a versatile tool in research laboratories. Additionally, NP40 Lysis Buffer is often used in studying protein-protein interactions and in preparing samples for downstream analytical techniques.
Product Specification:
Test Specification
APPEARANCE Colorless transparent, non precipitated liquid
ConcentrationTris 50mM
ConcentrationNaCl 150mM
Infrared spectrum Conforms to Structure
NP-40 1
INHIBITOR Pass
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
50.000 10-20 days $73.92
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-
100.000 10-20 days $114.62
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-
250.000 10-20 days $232.56
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-
1000.000 10-20 days $642.87
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NP40 Lysis Buffer
NP40 Lysis Buffer is widely used in molecular biology and biochemistry for cell lysis and protein extraction. It is particularly effective for isolating proteins from cell membranes and cytoplasm due to its ability to disrupt lipid bilayers while maintaining protein integrity. This buffer is commonly employed in immunoprecipitation, Western blotting, and enzyme activity assays, as it solubilizes proteins without denaturing them. It is also suitable for extracting nuclear and cytoplasmic proteins from cultured cells, making it a versatile tool in research laboratories. Additionally, NP40 Lysis Buffer is often used in studying protein-protein interactions and in preparing samples for downstream analytical techniques.
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