4-Nitrophenyl Phosphate Di(tris) Salt Hydrate [Substrate for Phosphatase]

>90.0%(T)

  • Product Code: 95977
  CAS:    68189-42-4
Molecular Weight: 461.36(as Anhydrous) g./mol Molecular Formula: C₆H₆NO₆PC₄H₁₁NO₃₂H₂O
EC Number: MDL Number: MFCD00037009
Melting Point: Boiling Point:
Density: Storage Condition: -20ºC
Product Description: This compound is widely used as a substrate in biochemical assays to measure the activity of phosphatase enzymes. When phosphatases act on it, they cleave the phosphate group, releasing 4-nitrophenol, which is yellow and can be easily quantified using spectrophotometry at around 405 nm. This makes it a valuable tool in research and diagnostic labs for studying enzyme kinetics, screening inhibitors, and assessing phosphatase activity in various biological samples. Its stability and sensitivity contribute to its effectiveness in high-throughput screening and enzyme-linked assays.
Product Specification:
Test Specification
APPEARANCE Very pale yellow - Pale reddish yellow Crystal - Powder
PURITY 90-100
Infrared spectrum Conforms to Structure
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
1.000 10-20 days $73.92
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5.000 10-20 days $250.00
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4-Nitrophenyl Phosphate Di(tris) Salt Hydrate [Substrate for Phosphatase]
This compound is widely used as a substrate in biochemical assays to measure the activity of phosphatase enzymes. When phosphatases act on it, they cleave the phosphate group, releasing 4-nitrophenol, which is yellow and can be easily quantified using spectrophotometry at around 405 nm. This makes it a valuable tool in research and diagnostic labs for studying enzyme kinetics, screening inhibitors, and assessing phosphatase activity in various biological samples. Its stability and sensitivity contribute to its effectiveness in high-throughput screening and enzyme-linked assays.
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