Tris-Glycine-SDS PAGE Running Buffer
10X
- Product Code: 97698
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Density: | Storage Condition: | 2-8°C |
Product Description:
Used primarily in protein electrophoresis, it facilitates the separation of proteins based on their molecular weight. The buffer provides the necessary ionic environment for proteins to migrate through the polyacrylamide gel under an electric field. SDS (sodium dodecyl sulfate) in the buffer denatures proteins, giving them a uniform charge-to-mass ratio, ensuring separation is based solely on size. This buffer is essential in techniques like SDS-PAGE, widely employed in molecular biology and biochemistry for protein analysis, purity checks, and molecular weight determination. It is also used in Western blotting as a transfer buffer to move proteins from gels to membranes for further detection.
Product Specification:
Test | Specification |
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Performance test | PASS |
APPERANCE | Liquid |
Sizes / Availability / Pricing:
Size (g) | Availability | Price | Quantity |
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100.000 | 10-20 days | ฿2,080.00 |
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250.000 | 10-20 days | ฿4,180.00 |
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Tris-Glycine-SDS PAGE Running Buffer
Used primarily in protein electrophoresis, it facilitates the separation of proteins based on their molecular weight. The buffer provides the necessary ionic environment for proteins to migrate through the polyacrylamide gel under an electric field. SDS (sodium dodecyl sulfate) in the buffer denatures proteins, giving them a uniform charge-to-mass ratio, ensuring separation is based solely on size. This buffer is essential in techniques like SDS-PAGE, widely employed in molecular biology and biochemistry for protein analysis, purity checks, and molecular weight determination. It is also used in Western blotting as a transfer buffer to move proteins from gels to membranes for further detection.
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