TMB Liquid substrate system

peroxidase substrate

  • Product Code: 98067
  Alias:    3,3′,5,5′-tetramethylbenzidine solution
  Properties:    3,3′,5,5′-Tetramethylbenzidine solution, to be used with T820899
Molecular Weight: Molecular Formula:
EC Number: MDL Number:
Melting Point: Boiling Point:
Density: Storage Condition: 2~8℃
Product Description: The TMB Liquid Substrate System is widely used in immunoassays, particularly in ELISA (Enzyme-Linked Immunosorbent Assay) procedures. It serves as a chromogenic substrate for detecting horseradish peroxidase (HRP) activity. When HRP is present, it catalyzes the oxidation of TMB, resulting in a color change from colorless to blue. This color change can be measured spectrophotometrically, providing a quantitative assessment of the target analyte. The system is valued for its high sensitivity, low background noise, and compatibility with various detection methods, making it a preferred choice in clinical diagnostics, research laboratories, and biopharmaceutical applications. Additionally, the reaction can be stopped with an acid, turning the solution yellow, which further enhances the ease of measurement.
Product Specification:
Test Specification
SUBSTRATE650NM 0.04
APPEARANCE Colorless to pale blue or pale yellow or pale green liquid
UV detection290nm detection
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
25.000 10-20 days £16.28
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100.000 10-20 days £46.82
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500.000 10-20 days £144.30
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2500.000 10-20 days £569.50
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TMB Liquid substrate system
The TMB Liquid Substrate System is widely used in immunoassays, particularly in ELISA (Enzyme-Linked Immunosorbent Assay) procedures. It serves as a chromogenic substrate for detecting horseradish peroxidase (HRP) activity. When HRP is present, it catalyzes the oxidation of TMB, resulting in a color change from colorless to blue. This color change can be measured spectrophotometrically, providing a quantitative assessment of the target analyte. The system is valued for its high sensitivity, low background noise, and compatibility with various detection methods, making it a preferred choice in clinical diagnostics, research laboratories, and biopharmaceutical applications. Additionally, the reaction can be stopped with an acid, turning the solution yellow, which further enhances the ease of measurement.
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