p-Nitrophenyl-β-D-galactopyranoside (pNPG)
99%
- Product Code: 98163
Alias:
p-Nitrophenyl-β-D-galactopyranoside (PNPG) ; 4-Nitrophenyl-β-D-galactopyranoside
CAS:
3150-24-1
| Molecular Weight: | 301.25 g./mol | Molecular Formula: | C₁₂H₁₅NO₈ |
|---|---|---|---|
| EC Number: | 221-584-5 | MDL Number: | MFCD00063256 |
| Melting Point: | 178-182 °C | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
p-Nitrophenyl-β-D-galactopyranoside (pNPG) is widely used as a substrate in enzymatic assays to measure β-galactosidase activity. When β-galactosidase cleaves pNPG, it releases p-nitrophenol, a yellow-colored compound that can be easily quantified using spectrophotometry at 405 nm. This makes pNPG a valuable tool in molecular biology and biochemistry for studying enzyme kinetics, screening for enzyme inhibitors, and monitoring gene expression in systems like the lac operon. Additionally, it is employed in diagnostic tests to detect bacterial contamination, particularly in food and water samples, by identifying β-galactosidase-producing microorganisms. Its high sensitivity and simplicity make it a preferred choice in both research and industrial applications.
Product Specification:
| Test | Specification |
|---|---|
| APPEARANCE | WHITE OR LIGHT YELLOW POWDER |
| PURITY | 98.5-100 |
| INFRARED SPECTRUM | CONFORMS TO STRUCTURE |
| SPECIFIC ROTATIONA20DC1H2O | -88--80 |
| WATER BY KARL FISCHER | 0-6 |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | ฿2,700.00 |
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| 5.000 | 10-20 days | ฿8,990.00 |
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| 25.000 | 10-20 days | ฿18,880.00 |
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p-Nitrophenyl-β-D-galactopyranoside (pNPG)
p-Nitrophenyl-β-D-galactopyranoside (pNPG) is widely used as a substrate in enzymatic assays to measure β-galactosidase activity. When β-galactosidase cleaves pNPG, it releases p-nitrophenol, a yellow-colored compound that can be easily quantified using spectrophotometry at 405 nm. This makes pNPG a valuable tool in molecular biology and biochemistry for studying enzyme kinetics, screening for enzyme inhibitors, and monitoring gene expression in systems like the lac operon. Additionally, it is employed in diagnostic tests to detect bacterial contamination, particularly in food and water samples, by identifying β-galactosidase-producing microorganisms. Its high sensitivity and simplicity make it a preferred choice in both research and industrial applications.
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