HPLC Analysis of Ectoin Purity
- Product Code: 35668
Reverse-phase HPLC-UV/PDA method for ectoin purity determination by relative peak area normalization.
description Sample Requirement
Ectoin powder, 0.1 g per run.
insert_drive_file Report of Previously Tested Sample
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timeline Service Steps
| Step | Procedure | Expected Result |
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1
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Prepare 0.025... |
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Clear buffer solution.
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2
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Mix buffer... |
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Stable mobile phase for isocratic run.
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3
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Prepare 500... |
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Clear calibration solution covering five levels.
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4
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Prepare sample... |
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Clear sample extract ready for chromatography.
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5
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Run HPLC... |
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Ectoin retention time ~8 min with tailing factor <2.
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6
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Calculate purity... |
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Purity expressed as percentage with acceptable precision.
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