Determination of total Antioxidant Activity using DPPH Assay
- Product Code: 30978
Antioxidant activity was measured with the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay by UV-Vis spectrophotometer which is a common method to evaluate the ability of compounds to scavenge free radicals. This assay was widely applied to assess the antioxidant properties of natural products, extracts, and compounds.
insert_drive_file Report of Previously Tested Sample
timeline ขั้นตอนการให้บริการ
| ขั้นตอน | ขั้นตอน | ผลลัพธ์ที่คาดหวัง |
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1
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Weigh 10... |
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A clear extract, ready for the DPPH assay. |
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2
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Prepare 1... |
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A purple DPPH solution in ethanol, stable for the duration of the assay.
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3
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For each... |
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Reaction mixture containing DPPH and antioxidant sample, ready for incubation. |
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4
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Add 230... |
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test sample |
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5
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Add 20... |
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sample and reagent mixture and start reaction |
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6
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Incubate at... |
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discoloration |
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7
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Absorbance is... |
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absorbance of test reaction |
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8
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Each measurement... |
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ensure test protocol |
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9
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The percentage... |
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% inhibition |
คุณจะได้รับรายงานสำหรับ %inhibition , absorbance at 517nm ฟิลด์เมื่อเราให้บริการนี้
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