Tannins are water soluble polyphenols. Tea leaves are abundant natural sources of polyphenols. polyphenols are examples of the most commonly used natural antioxidants, anticancer, antimicrobial, reducing cardiovascular diseases and anti-inflammatory. Follin–Dennis method, which is the one of the oldest colorimetric procedures that's determines total soluble phenolics and especially to determine the tannin content and the absorbance of blue coloration was measured using UV-VIS spectrophotometer the intensity of which is proportional to the number of tannins at 725 nm. Sample Requirement: 30µL