UV-VIS Total Flavonoids Content (Aluminium Chloride Colorimetric)
- Product Code: 30977
Analysis of Total Phenolic Content using Aluminium Chloride Colorimetric
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UV-VIS Total Flavonoids Content
Analysis of Total Phenolic Content using Aluminium Chloride Colorimetric
Measuring the total flavonoid content of a sample using the aluminum chloride colorimetric assay with UV-Vis spectrophotometry is a common method for quantifying flavonoids, a group of naturally occurring polyphenolic compounds found in various plants and foods. Here's a general protocol for performing a UV-Vis total flavonoid content assay using the aluminum chloride reagent
timeline ขั้นตอนการให้บริการ
| ขั้นตอน | ขั้นตอน | ผลลัพธ์ที่คาดหวัง |
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1
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Weigh 10... |
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Clear solution; if insoluble, sonicate or heat gently <40°C. |
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2
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Prepare quercetin... |
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Clear standards ready for assay.
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3
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Into labeled... |
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sample |
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4
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Add 1.8... |
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sample and AlCl3 mixture |
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5
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After mixing,... |
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yellow color development |
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6
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The absorbance... |
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absorbance of reaction |
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7
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The flavonoid... |
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flavonoid content |
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