Folin-Ciocalteu Assay for Total Phenolic Content (UV-Vis)
Spectrophotometric method measuring total phenolics in plant extracts via reaction with Folin-Ciocalteu reagent, absorbance at 765 nm, expressed as gallic acid equivalents.
Sample Requirement:
plant extract (lyophilized or dry) · 0.1 · grams
Sample Count:
Accepts exactly 1 sample.
Phenolic, Flavonoids, Carotenoids
Phenolic, Flavonoids Measurement using UV
UV-VIS Total Flavonoids Content (Aluminum Chloride Colorimetric Assay)
UV-Vis spectrophotometric assay for total flavonoid content in plant extracts via complexation with aluminum chloride, quantified as quercetin equivalents at 420 nm.
Sample Requirement:
dry plant extract · 10 · mg
Sample Count:
Accepts exactly 1 sample.
Determination of Tannin Content (Folin-Denis Method)
Tannins are water soluble polyphenols. Tea leaves are abundant natural sources of polyphenols. polyphenols are examples of the most commonly used natural antioxidants, anticancer, antimicrobial, reducing cardiovascular diseases and anti-inflammatory. Follin–Dennis method, which is the one of the oldest colorimetric procedures that's determines total soluble phenolics and especially to determine the tannin content and the absorbance of blue coloration was
measured using UV-VIS spectrophotometer the intensity of which is proportional to the number of tannins at 725 nm.
Sample Requirement:
30µL
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