Obtaining 10% Ammonium per Sulfate (APS) solution.

Obtaining 10% Sodium dodecyl sulfate (SDS) solution.

Obtaining1M Tris Hydrochloric Acid , pH 6.8.

Obtaining1.5M Tris Hydrochloric Acid, pH 8.8.

Obtaining 30% Acrylamide solution.

Obtaining 10X Laemmli electrode buffer solution.

Obtaining 1X Laemmli electrode buffer (working) solution.

Obtaining 2X Sample loading buffer (with reducing agent) solution.

Obtaining 10% of separating gel.

Obtaining 12.5% separating gel.

Obtaining 15% separating gel.

Obtaining 18% separating gel .

Obtaining 20% separating gel.

Obtaining Stacking gel .

Obtaining fixing solution .

Obtaining Staining Solution.

Obtaining Destaining Solution.

Requirement tools .

Reaction occurs fold protein to unfold protein .  

Formation of a blue-colored complex between the test sample and reaction solution.

To run the Molecular Size of Sample.

To run the Molecular Size of Sample.

To fix color of gel layer before dying.

To dye the gel layer.

Obtaining the clear gel layer.

Obtaining the approximate protein molecular weight in unknown sample.