pH Measurement Report
pH measurement is a fundamental analytical technique that provides valuable information about the chemical properties of a solution. It is essential for ensuring product quality, understanding chemical reactions, and maintaining environmental health and safety.
Sample Requirement:
10g
Sample Count:
Accepts exactly 1 sample.
Anti alpha glucosidase assay
Under specified conditions (pH = 6.8; T = 37 °C), α -glucosidase will catalyze the conversion of the substrate 4-nitrophenyl-α-D-glucopyranoside to α-D-glucopyranoside and p-nitrophenol, as shown below (Equation (1)). The yellow colour of the latter product is measured spectrophotometrically at 405 nm.
[PNPG + α-glucosidase] → α-D-glucopyranoside + PNP (yellow)]
Alpha-glucosidase inhibitors inhibit the absorption of carbohydrates from the small intestine. They competitively inhibit enzymes that convert complex nonabsorbable carbohydrates into simple absorbable carbohydrates. These enzymes include glucoamylase, sucrase, maltase, and isomaltase. By delaying carbohydrate absorption, they reduce the rise in postprandial blood glucose concentrations by about 3mmol/L.
Sample Requirement:
1 g
Protein denaturation inhibition
The protein denaturation inhibition assay is essential for evaluating the anti-inflammatory properties of plant extracts and compounds. By assessing the ability of a sample to prevent or reduce the denaturation of egg albumin, researchers can identify potential anti-inflammatory agents that stabilize protein structures, thereby mitigating inflammation and tissue damage. This assay is valuable in pharmaceutical development, natural product research, and the formulation of anti-inflammatory therapies. The assay employs egg albumin as a model protein whose denaturation is induced by heat. Anti-inflammatory agents are hypothesized to stabilize the protein structure, preventing denaturation. By measuring the extent of albumin denaturation through absorbance at 660 nm, the method provides a reli
Density Measurement (Liquid, Solid)
The upward buoyant force is exactly equal to the weight of the fluid that the object displaced. Because the liquid is pushing the glass ball up, the ball will physically appear to weigh less when it is submerged in the liquid than it does when it is hanging in the open air.
Sample Requirement:
250ml
Sample Count:
Accepts exactly 1 sample.
Broth Microdilution Minimum Inhibitory Concentration (MIC) Test for Propionibacterium acnes
Broth microdilution method to determine MIC of test antimicrobial agents against P. acnes (now Cutibacterium acnes) under anaerobic conditions.
Sample Requirement:
Test antimicrobial agent (pure compound or stock solution), 50 mg
Sample Count:
Accepts exactly 1 sample.
Determination of total Antioxidant Activity IC50 using ABTS Assay
The antioxidant activity of the test sample was measured with the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay by Microplate Reader which is the most popular method to evaluate the ability of compounds to scavenge free radicals. The ABTS assay was adapted to measure the antioxidant activity of natural products, extracts, and compounds.
Sample Count:
Accepts exactly 1 sample.
Disk Diffusion Assay for Antimicrobial Activity against Escherichia coli
Agar-based method measuring inhibition zone diameters around disks impregnated with test substance on E. coli lawn to assess antimicrobial activity.
Sample Requirement:
Test substance solution 10 mL
Sample Count:
Accepts exactly 1 sample.
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