UV-VIS Anti-Elastase Assay

  • Product Code: 31936
Spectrophotometric assay for inhibitory activity of skin extracts against porcine pancreatic elastase using N-succinyl-Ala-Ala-Ala-p-nitroanilide substrate, measured at 410 nm.
฿2,990.00

label ชื่อตัวอย่าง

info Please add exactly one sample

assignment คำแนะนำ

info โปรดระบุข้อกำหนดหรือเงื่อนไขเฉพาะสำหรับการทดสอบ

description Sample Requirement

skin tissue · 1 · g

insert_drive_file Report of Previously Tested Sample

Select a tab and click View to open the report.

timeline ขั้นตอนการให้บริการ

ขั้นตอน ขั้นตอน ผลลัพธ์ที่คาดหวัง
1

Weigh 10...

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Homogenized suspension.

2

Add 160...

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buffer

3

Add 20...

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test

4

Add 20...

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test sample with enzyme mixture

5

Add 20...

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test sample, enzyme and substrate mixture

6

Incubate at...

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enzyme-substrate reaction

7

Absorbance is...

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absorbance of reaction

8

Each measurement...

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ensure test protocol

9

The percentage...

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% inhibition

UV-VIS Anti-Elastase Assay
Spectrophotometric assay for inhibitory activity of skin extracts against porcine pancreatic elastase using N-succinyl-Ala-Ala-Ala-p-nitroanilide substrate, measured at 410 nm. Sample Requirement: skin tissue · 1 · g Sample Count: Accepts exactly 1 sample.
Spectrophotometric assay for inhibitory activity of skin extracts against porcine pancreatic elastase using N-succinyl-Ala-Ala-Ala-p-nitroanilide substrate, measured at 410 nm. Sample Requirement: skin tissue · 1 · g Sample Count: Accepts exactly 1 sample.
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Step Procedure Expected Result
1

Weigh 10...

Homogenized suspension.

2

Add 160...

buffer

3

Add 20...

test

4

Add 20...

test sample with enzyme mixture

5

Add 20...

test sample, enzyme and substrate mixture

6

Incubate at...

enzyme-substrate reaction

7

Absorbance is...

absorbance of reaction

8

Each measurement...

ensure test protocol

9

The percentage...

% inhibition