UV-VIS Anti-Elastase Assay
- Product Code: 31936
Spectrophotometric assay for inhibitory activity of skin extracts against porcine pancreatic elastase using N-succinyl-Ala-Ala-Ala-p-nitroanilide substrate, measured at 410 nm.
description Sample Requirement
skin tissue · 1 · g
insert_drive_file Report of Previously Tested Sample
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
|
1
|
Weigh 10... |
check_circle_outline
Homogenized suspension. |
|
2
|
Add 160... |
check_circle_outline
buffer |
|
3
|
Add 20... |
check_circle_outline
test |
|
4
|
Add 20... |
check_circle_outline
test sample with enzyme mixture |
|
5
|
Add 20... |
check_circle_outline
test sample, enzyme and substrate mixture |
|
6
|
Incubate at... |
check_circle_outline
enzyme-substrate reaction |
|
7
|
Absorbance is... |
check_circle_outline
absorbance of reaction |
|
8
|
Each measurement... |
check_circle_outline
ensure test protocol |
|
9
|
The percentage... |
check_circle_outline
% inhibition |
Cart
No products
Subtotal:
0.00
Total
0.00
THB
Loading Researcher Network details...
