UHPLC-DAD Method for Caffeine Quantitation in Herbal Extract
- Product Code: 31493
Ultra-high-performance liquid chromatography with diode array detection for caffeine determination in powdered herbal extracts using reference standard.
Sample Requirement:
powdered herbal extract · 0.5 · g
description ภาพรวมบริการ
Ultra-high-performance liquid chromatography with diode array detection for caffeine determination in powdered herbal extracts using reference standard.
Sample Requirement:
powdered herbal extract · 0.5 · g
timeline ขั้นตอนการให้บริการ
| ขั้นตอน | ขั้นตอน | ผลลัพธ์ที่คาดหวัง |
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1
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Accurately weigh... |
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Precise mass recorded (±0.1 mg).
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2
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Add 50... |
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Sample fully dispersed and extracted.
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3
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Centrifuge at... |
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Clear supernatant separated from pellet.
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4
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Transfer ~5... |
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Particle-free filtrate ready for injection.
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5
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Prepare caffeine... |
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Linear calibration curve (r² > 0.999) at 272 nm.
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6
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Run UHPLC-DAD:... |
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Caffeine peak at ~3-4 min, quantified vs. curve.
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7
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Include method... |
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QC passes (±15% RSD); report LOD/LOQ from curve.
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