UHPLC-DAD Method for Caffeine Quantitation in Herbal Extract

  • Product Code: 31493
Ultra-high-performance liquid chromatography with diode array detection for caffeine determination in powdered herbal extracts using reference standard.
฿3,000.00

label Sample Names

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assignment Instructions

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description Sample Requirement

powdered herbal extract · 0.5 · g

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timeline Service Steps

Step Procedure Expected Result
1
Accurately weigh...
check_circle_outline Precise mass recorded (±0.1 mg).
2
Add 50...
check_circle_outline Sample fully dispersed and extracted.
3
Centrifuge at...
check_circle_outline Clear supernatant separated from pellet.
4
Transfer ~5...
check_circle_outline Particle-free filtrate ready for injection.
5
Prepare caffeine...
check_circle_outline Linear calibration curve (r² > 0.999) at 272 nm.
6
Run UHPLC-DAD:...
check_circle_outline Caffeine peak at ~3-4 min, quantified vs. curve.
7
Include method...
check_circle_outline QC passes (±15% RSD); report LOD/LOQ from curve.

UHPLC-DAD Method for Caffeine Quantitation in Herbal Extract
Ultra-high-performance liquid chromatography with diode array detection for caffeine determination in powdered herbal extracts using reference standard. Sample Requirement: powdered herbal extract · 0.5 · g
Ultra-high-performance liquid chromatography with diode array detection for caffeine determination in powdered herbal extracts using reference standard. Sample Requirement: powdered herbal extract · 0.5 · g
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Service Steps
Step Procedure Expected Result
1 Accurately weigh... Precise mass recorded (±0.1 mg).
2 Add 50... Sample fully dispersed and extracted.
3 Centrifuge at... Clear supernatant separated from pellet.
4 Transfer ~5... Particle-free filtrate ready for injection.
5 Prepare caffeine... Linear calibration curve (r² > 0.999) at 272 nm.
6 Run UHPLC-DAD:... Caffeine peak at ~3-4 min, quantified vs. curve.
7 Include method... QC passes (±15% RSD); report LOD/LOQ from curve.