Detection of Salmonella

Detection of Salmonella
  • Product Code: 127388
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
฿800.00

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Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams

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ขั้นตอน ขั้นตอน ผลลัพธ์ที่คาดหวัง
1

Aseptically transfer...
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Sample prepared for homogenization and neutralization. Turbid growth indicating enrichment.
2

Streak an...
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From SCDA/TSA, select colonies that fit an enteric Gram-negative rod profile:

  • smooth colony type,
  • Gram-negative rod on smear,
  • oxidase negative preferred. ISO 18415 directs Gram stain first and oxidase testing for Gram-negative rods, then a suitable identification path.
3

Pick suspicious...
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Black-centered colonies on XLD indicating presumptive Salmonella (30-300 colonies ideal).
4

Count colonies...
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Reported Salmonella CFU per gram.
Detection of Salmonella
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
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Service Steps
Step Procedure Expected Result
1

Aseptically transfer...

Sample prepared for homogenization and neutralization. Turbid growth indicating enrichment.
2

Streak an...

From SCDA/TSA, select colonies that fit an enteric Gram-negative rod profile:

  • smooth colony type,
  • Gram-negative rod on smear,
  • oxidase negative preferred. ISO 18415 directs Gram stain first and oxidase testing for Gram-negative rods, then a suitable identification path.
3

Pick suspicious...

Black-centered colonies on XLD indicating presumptive Salmonella (30-300 colonies ideal).
4

Count colonies...

Reported Salmonella CFU per gram.