Detection of Salmonella

Detection of Salmonella
  • Product Code: 127388
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
฿800.00

science Sample Names

assignment Instructions

info Please include any specific requirements or conditions for testing

description Service Overview

Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams

timeline Service Steps

Step Procedure Expected Result
1

Aseptically transfer...
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Sample prepared for homogenization and neutralization. Turbid growth indicating enrichment.
2

Streak an...
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From SCDA/TSA, select colonies that fit an enteric Gram-negative rod profile:

  • smooth colony type,
  • Gram-negative rod on smear,
  • oxidase negative preferred. ISO 18415 directs Gram stain first and oxidase testing for Gram-negative rods, then a suitable identification path.
3

Pick suspicious...
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Black-centered colonies on XLD indicating presumptive Salmonella (30-300 colonies ideal).
4

Count colonies...
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Reported Salmonella CFU per gram.
Detection of Salmonella
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
Quantitative estimation of Salmonella spp. concentration in food and cosmetic samples using pre-enrichment, selective enrichment, and XLD agar plating. Sample Requirement: 10 grams
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Service Steps
Step Procedure Expected Result
1

Aseptically transfer...

Sample prepared for homogenization and neutralization. Turbid growth indicating enrichment.
2

Streak an...

From SCDA/TSA, select colonies that fit an enteric Gram-negative rod profile:

  • smooth colony type,
  • Gram-negative rod on smear,
  • oxidase negative preferred. ISO 18415 directs Gram stain first and oxidase testing for Gram-negative rods, then a suitable identification path.
3

Pick suspicious...

Black-centered colonies on XLD indicating presumptive Salmonella (30-300 colonies ideal).
4

Count colonies...

Reported Salmonella CFU per gram.