Flavonoids Content Assay Kit
enzyme labeling method
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| พารามิเตอร์การทดสอบ | ข้อมูลจำเพาะ |
|---|---|
| Performance test | PASS |
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Plant Flavonoid Content Assay Kit - Microplate Method
Product Information
| Product Code | 112395 |
|---|---|
| Product Name | Plant Flavonoid Content Assay Kit - Microplate Method |
| Assay Size | 100T |
Product Introduction
Flavonoids are polyphenolic compounds and plant secondary metabolites. Their reported biological activities include anti-inflammatory, antibacterial, lipid-lowering, hydroxyl radical scavenging, and cancer prevention effects.
In an alkaline nitrite solution, flavonoids form a red complex with aluminum ions. This complex has a characteristic absorption peak at 510 nm. The flavonoid content of a sample can be calculated by measuring the absorbance of the sample extract at 510 nm.
Actual readings may vary under different testing conditions and with different instruments.
Package Contents
| Component Code | Component | Quantity |
|---|---|---|
| BR5000386.1 | Reagent I | 1 tube |
| BR5000386.2 | Reagent II | 1 tube |
| BR5000386.3 | Reagent III | 1 bottle |
| BR5000386.m | Instruction Manual | 1 copy |
Quality and Safety Information
| Raw Material or Packaging Name | Quality Standard | Main Toxicity |
|---|---|---|
| Reagent I | -- | -- |
| Reagent II | -- | -- |
| Reagent III | -- | -- |
Transportation and Storage
| Transportation | Transport with ice packs. |
|---|---|
| Storage | Store at 2-8℃. Shelf life: 180 days. |
Instructions for Use
1. Flavonoid Extraction
- Dry the sample to constant weight.
- Pulverize the dried sample and pass it through a 40 mesh sieve.
- Weigh approximately 0.02 g of sample.
- Add 2 mL of 60% ethanol.
- Extract by shaking at 60℃ for 2 h.
- Centrifuge at 10000 g and 25℃ for 10 min.
- Collect the supernatant for testing.
2. Assay Procedure
- Preheat the microplate reader for at least 30 min and set the wavelength to 510 nm.
- Add the components according to the table below.
| Component | Blank Tube | Assay Tube |
|---|---|---|
| Sample test solution (μL) | - | 108 |
| Distilled water (μL) | 108 | - |
| Reagent I (μL) | 6 | 6 |
| Mix well and let stand at room temperature for 5 min. | ||
| Reagent II (μL) | 6 | 6 |
| Mix well and let stand at room temperature for 5 min. | ||
| Reagent III (μL) | 80 | 80 |
| Mix well and let stand at 25℃ for 15 min. | ||
Measure the absorbance at 510 nm after incubation. Calculate ΔA as follows:
ΔA = A assay - A blank
Prepare only one blank tube.
3. Calculation of Flavonoid Content
Standard curve:
y = 2.51x + 0.0007, R2= 0.9996
Flavonoid content (mg/g, dry weight):
(ΔA - 0.0007) ÷ 2.51 ÷ (W ÷ V total sample) = 0.797 × (ΔA - 0.0007) ÷ W
| Symbol | Description | Value |
|---|---|---|
| V sample total | Volume of extraction solution added | 2 mL |
| V sample | Sample volume in the reaction | 0.108 mL |
| W | Sample mass | g |
Precautions
- This 100T product can test 96 samples.
- Before the formal assay, select 2-3 samples with large expected differences for a preliminary assay.
- Self-prepared laboratory supplies and instruments include: balance, oven, grinder, sieve, ultrasonic disruptor, 60% ethanol, centrifuge, microplate reader, 96-well plate, and distilled water.
- If the OD value is greater than 0.8, dilute the sample appropriately before measurement. The calculation formula should be multiplied by the dilution factor.
- The minimum detection limit is 10 μg/g.
- Measure immediately after color development is completed. After 2 hours, the absorbance value will decrease.
Visual Reference
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