Flavonoids Content Assay Kit
enzyme labeling method
Reagent
Code: #112395
blur_circular Chemical Specifications
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Storage & Handling
Storage
2-8℃
description Product Description
The Flavonoids Content Assay Kit is widely used in research and quality control to measure flavonoid levels in various samples, such as plant extracts, food products, and dietary supplements. It is essential for studying the antioxidant properties of flavonoids, which are crucial for understanding their health benefits, including anti-inflammatory, anti-cancer, and cardiovascular protective effects. In the food industry, this kit helps ensure the nutritional value and consistency of products rich in flavonoids, such as teas, wines, and fruits. Additionally, it supports agricultural research by evaluating flavonoid content in crops to enhance their nutritional quality and stress resistance. The kit is also utilized in pharmaceutical development to standardize flavonoid-based formulations for therapeutic use. Its simplicity and accuracy make it a valuable tool for scientists and industry professionals.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Performance test | PASS |
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Plant Flavonoid Content Assay Kit - Microplate Method
Product Information
| Product Code | 112395 |
|---|---|
| Product Name | Plant Flavonoid Content Assay Kit - Microplate Method |
| Assay Size | 100T |
Product Introduction
Flavonoids are polyphenolic compounds and plant secondary metabolites. Their reported biological activities include anti-inflammatory, antibacterial, lipid-lowering, hydroxyl radical scavenging, and cancer prevention effects.
In an alkaline nitrite solution, flavonoids form a red complex with aluminum ions. This complex has a characteristic absorption peak at 510 nm. The flavonoid content of a sample can be calculated by measuring the absorbance of the sample extract at 510 nm.
Actual readings may vary under different testing conditions and with different instruments.
Package Contents
| Component Code | Component | Quantity |
|---|---|---|
| BR5000386.1 | Reagent I | 1 tube |
| BR5000386.2 | Reagent II | 1 tube |
| BR5000386.3 | Reagent III | 1 bottle |
| BR5000386.m | Instruction Manual | 1 copy |
Quality and Safety Information
| Raw Material or Packaging Name | Quality Standard | Main Toxicity |
|---|---|---|
| Reagent I | -- | -- |
| Reagent II | -- | -- |
| Reagent III | -- | -- |
Transportation and Storage
| Transportation | Transport with ice packs. |
|---|---|
| Storage | Store at 2-8℃. Shelf life: 180 days. |
Instructions for Use
1. Flavonoid Extraction
- Dry the sample to constant weight.
- Pulverize the dried sample and pass it through a 40 mesh sieve.
- Weigh approximately 0.02 g of sample.
- Add 2 mL of 60% ethanol.
- Extract by shaking at 60℃ for 2 h.
- Centrifuge at 10000 g and 25℃ for 10 min.
- Collect the supernatant for testing.
2. Assay Procedure
- Preheat the microplate reader for at least 30 min and set the wavelength to 510 nm.
- Add the components according to the table below.
| Component | Blank Tube | Assay Tube |
|---|---|---|
| Sample test solution (μL) | - | 108 |
| Distilled water (μL) | 108 | - |
| Reagent I (μL) | 6 | 6 |
| Mix well and let stand at room temperature for 5 min. | ||
| Reagent II (μL) | 6 | 6 |
| Mix well and let stand at room temperature for 5 min. | ||
| Reagent III (μL) | 80 | 80 |
| Mix well and let stand at 25℃ for 15 min. | ||
Measure the absorbance at 510 nm after incubation. Calculate ΔA as follows:
ΔA = A assay - A blank
Prepare only one blank tube.
3. Calculation of Flavonoid Content
Standard curve:
y = 2.51x + 0.0007, R2= 0.9996
Flavonoid content (mg/g, dry weight):
(ΔA - 0.0007) ÷ 2.51 ÷ (W ÷ V total sample) = 0.797 × (ΔA - 0.0007) ÷ W
| Symbol | Description | Value |
|---|---|---|
| V sample total | Volume of extraction solution added | 2 mL |
| V sample | Sample volume in the reaction | 0.108 mL |
| W | Sample mass | g |
Precautions
- This 100T product can test 96 samples.
- Before the formal assay, select 2-3 samples with large expected differences for a preliminary assay.
- Self-prepared laboratory supplies and instruments include: balance, oven, grinder, sieve, ultrasonic disruptor, 60% ethanol, centrifuge, microplate reader, 96-well plate, and distilled water.
- If the OD value is greater than 0.8, dilute the sample appropriately before measurement. The calculation formula should be multiplied by the dilution factor.
- The minimum detection limit is 10 μg/g.
- Measure immediately after color development is completed. After 2 hours, the absorbance value will decrease.
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